Effect of soluble endoglin on cholesterol metabolism in the liver of high-fat diet-fed transgenic mice

Kristýna Hofmanová Effect of soluble endoglin on cholesterol metabolism in the liver of high-fat diet-fed transgenic mice Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Pharmacy Background: Increased plasma concentrations of soluble endoglin (sENG) are observed in metabolic deseases like diabetes mellitus type 2 or hypercholesterolemia. It has already been shown that sEng influence the cholesterol metabolism i healthy mice fed standard laboratory diet. However, the effect of sENG on cholesterol metabolism in combination with high fat diet (HFD) is not known yet. The aim of this diploma thesis was to elucidate the influece of the sENG on the meabolism of cholesterol in the liver of transgenic mice (Sol-Eng+ ), with liver demaged by NASH (Non-alcoholic steatohepatitis) induced by HFD. Methods: Three months old male mice (Sol-Eng+ ) with high plasma levels of sENG on the basis of the CBAxC57BL/6J and control "wild-type" mice with unspecified levels of soluble endoglin (n=8, in each group) we used in this study. The animals were divided into groups acording of the level of soluble endoglin and the type of diet: "wild-type" fed for six moths with standard laboratory diet (WTchow); "wild-type" with high fat diet (WTHFD) and high- plasma sENG group fed HFD for six months...