Saturation of genomic region implicated with resistance to Fusarium in chickpea

Trabajo presentado en la V Meeting Asociación Española de Leguminosas, AEL (Eucarpia International Symposium on Protein Crops), celebrada en Pontevedra del 4 al 7 de mayo de 2015.
Fusarium wilt, caused by Fusarium oxysporum Schlechtend: Fr. f. sp. ciceris (Padwick) Matuto & K. Sato, is the major soil-borne fungus affecting chickpea ( Cicer arietinum L.). The disease has been reported from almost all of the world’s chickpea growing areas. Resistance reaction to different races has oligogenic control being implicated from one to three genes. In order to saturate the area near the STMS (Sequence Tagged Microsatellite Sited) marker TA59 in LG2 (Linkage Group), highly associated with the resistance to fusarium, new STMS or gene specific markers with a known position in the genome of chickpea were selected. Four of the selected markers could be included in the genetic maps of two different RIL (Recombinant Inbred Lines) populations used in this study. In addition, pairs of NILs (Near Isogenic Lines) with resistance/susceptibility to fusarium were characterized using the same markers. Taking into account markers’s position in the physical map and genotypes in RILs, recombinant lines in this area were selected. Joining the information in RILs and NILs it was possible to select the area between 23224495..24088040 pb in chromosome 2 (Ca2) to search for candidate genes. In this window there are thirty genes and four of them could be considered candidate genes for their possible implication in resistance to fusarium.