DETERMINATION OF MERCURY IN LIVER OF MARINE TETRAPODS BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROMETRY AND INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY IN BIOLOGICAL SAMPLES: A SYSTEMATIC COMPARISON BETWEEN THE TWO TECHNIQUES

Made available in DSpace on 2021-06-25T12:40:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T14:36:37Z : No. of bitstreams: 1 S0100-40422021000100064.pdf: 351814 bytes, checksum: 4cae0f233c7cc9ac074ed30bb30901cd (MD5) This study compares the techniques cold vapour atomic fluorescence spectrometry (CV-AFS) and inductively coupled plasma mass spectrometry (ICP-MS) for the determination of Hg in biological samples (hepatic tissue from marine tetrapods). Acid digestions were performed for the samples and aliquots of them were analysed by ICP-MS and CV-AFS. To minimize the Hg memory effect in its determination by ICP-MS, gold (200 mu g L-1 in 5% v v(-1) HCl) was added to the sample digests. The detection limits were 0.02 mg kg(-1) for ICP-MS and 0.01 mg kg(-1) for CV-AFS. Accuracy was assessed by the calculation of the recovery obtained from TORT-3 (lobster hepatopancreas) certified reference material analysis. Accurate results were obtained for both CV-AFS and ICP-MS. The comparison of the results obtained for hepatic tissue with and without lyophilization showed a significant difference among them (at 95% of confidence level), evidencing losses of Hg in the lyophilization process. Univ Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil Univ Fed Amazonas, Dept Quim, BR-69067005 Manaus, Amazonas, Brazil Inst Nacl de Pesquisas da Amazonia, BR-69060001 Manaus, Amazonas, Brazil Univ Estadual Paulista, Ctr Estudos Ambientais, BR-13506900 Rio Claro, SP, Brazil