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Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

Authors :
Campos, F. S.
Franco, A. C.
Oliveira, M. T.
Firpo, R.
Strelczuk, G.
Fontoura, F. E.
Kulmann, M. I. R.
Maidana, Silvina Soledad
Romera, Sonia
Spilki, F. R.
Silva, A. D.
Hübner, S. O.
Roehe, P. M.
Source :
CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
Publication Year :
2014
Publisher :
Elsevier, 2014.

Abstract

Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon. Fil: Campos, F. S.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Franco, A. C.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Oliveira, M. T.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Firpo, R.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Strelczuk, G.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Fontoura, F. E.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Kulmann, M. I. R.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina Fil: Romera, Sonia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Spilki, F. R.. Universidade Feevale; Brasil Fil: Silva, A. D.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Hübner, S. O.. Universidade Federal de Pelotas; Brasil Fil: Roehe, P. M.. Universidade Federal do Rio Grande do Sul; Brasil. Instituto de Pesquisa Veterinária Desiderio Finamor; Brasil

Details

Language :
English
Database :
OpenAIRE
Journal :
CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
Accession number :
edsair.dedup.wf.001..307dfc318c3943bca8621dd47e2d2622