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High Prevalence and Diversity of Hepatitis Viruses in Suspected Cases of Yellow Fever in the Democratic Republic of Congo

Authors :
Makiala-Mandanda, S.
Le Gal, F.
Ngwaka-Matsung, N.
Ahuka-Mundeke, S.
Onanga, R.
Bivigou-Mboumba, B.
Pukuta-Simbu, E.
Gerber, A.
Abbate, J. L.
Mwamba, D.
Berthet, N.
Leroy, Eric
Muyembe-Tamfum, J. J.
Becquart, Pierre
Centre International de Recherches Médicales de Franceville (CIRMF)
Unité de modélisation mathématique et informatique des systèmes complexes [Bondy] (UMMISCO)
Université de Yaoundé I-Institut de la francophonie pour l'informatique-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Université Gaston Bergé (Saint-Louis, Sénégal)-Université Cadi Ayyad [Marrakech] (UCA)-Sorbonne Université (SU)-Institut de Recherche pour le Développement (IRD [France-Nord])
Source :
Journal of Clinical Microbiology, Journal of Clinical Microbiology, 2017, 55 (5), pp.1299-1312. ⟨10.1128/JCM.01847-16⟩
Publication Year :
2017
Publisher :
HAL CCSD, 2017.

Abstract

International audience; The majority of patients with acute febrile jaundice (>95%) identified through a yellow fever surveillance program in the Democratic Republic of Congo (DRC) test negative for antibodies against yellow fever virus. However, no etiological investigation has ever been carried out on these patients. Here, we tested for hepatitis A (HAV), hepatitis B (HBV), hepatitis C (HCV), hepatitis D (HDV), and hepatitis E (HEV) viruses, all of which can cause acute febrile jaundice, in patients included in the yellow fever surveillance program in the DRC. On a total of 498 serum samples collected from suspected cases of yellow fever from January 2003 to January 2012, enzyme-linked immunosorbent assay (ELISA) techniques were used to screen for antibodies against HAV (IgM) and HEV (IgM) and for antigens and antibodies against HBV (HBsAg and anti-hepatitis B core protein [HBc] IgM, respectively), HCV, and HDV. Viral loads and genotypes were determined for HBV and HVD. Viral hepatitis serological markers were diagnosed in 218 (43.7%) patients. The seroprevalences were 16.7% for HAV, 24.6% for HBV, 2.3% for HCV, and 10.4% for HEV, and 26.1% of HBV-positive patients were also infected with HDV. Median viral loads were 4.19 × 10 5 IU/ml for HBV (range, 769 to 9.82 × 10 9 IU/ml) and 1.4 × 10 6 IU/ml for HDV (range, 3.1 × 10 2 to 2.9 × 10 8 IU/ml). Genotypes A, E, and D of HBV and genotype 1 of HDV were detected. These high hepatitis prevalence rates highlight the necessity to include screening for hepatitis viruses in the yellow fever surveillance program in the DRC.

Details

Language :
English
ISSN :
00951137
Database :
OpenAIRE
Journal :
Journal of Clinical Microbiology, Journal of Clinical Microbiology, 2017, 55 (5), pp.1299-1312. ⟨10.1128/JCM.01847-16⟩
Accession number :
edsair.dedup.wf.001..2e8e960346b66aad26f8cc5f80ebc53f