Chromosomal localization of genes of interest for Solea senegalensis aquaculture using BAC clones

Senegalese sole is one of the most highly valued commercial species in Southern Europe. Nevertheless, there are still unresolved problems that adversely affect its cultivation and prevent the maximum benefit being obtained from its production. Knowing the position of genes of interest in the chromosomes facilitates the construction of linkage maps and allows the chromosomes to be assigned to physical maps obtained by sequencing. This study is an initial approach to the production of a chromosome map of S. senegalensis. The starting point is a gene library of cloned inserts in vectors capable of hosting fragments of large size: in our case, artificial chromosomes of bacteria (BAC). We have developed a protocol of single and double FISH techniques for localizing individual BAC, and have also performed double FISH for localizing two markers at the same time. Our results show the localization of BAC clones that contain genes related to the innate immune system that are important for the cultivation of this species, and genes and receptors of thyroid hormones involved in the metamorphosis of the sole. Thus the first chromosome map of S. senegalensis has been obtained. Lenguado senegalés es una de las especies más valoradas comerciales en el sur de Europa. Sin embargo, todavía hay problemas sin resolver que afectan negativamente a su cultivo y prevenir el beneficio máximo se obtiene a partir de su producción. El conocimiento de la posición de los genes de interés en los cromosomas facilita la construcción de mapas de ligamiento y permite a los cromosomas ser asignados a los mapas físicos obtenidos por secuenciación. Este estudio es una primera aproximación a la producción de un mapa cromosómico de S. senegalensis.