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Effect of Morphine-Sensitization in D2 Receptor Gene Expression in the Mice Brain in the Absence and Presence of Lithium Chloride

Authors :
Mehregan, H.
Sadeghizadeh, M.
Zarrindast, M. R.
Kayhan Azadmanesh
Jahanshahi, A.
Shirzad, H.
Saadati, M.
Source :
Cell Journal, Vol 12, Iss 3, Pp 395-404 (2010), Scopus-Elsevier
Publication Year :
2010
Publisher :
Royan Institute (ACECR), Tehran, 2010.

Abstract

Objective: In this study we have investigated the changes in D2 receptor expression levelin morphine-sensitized mice, in the absence and presence of lithium chloride (LiCl). Theresult would pave the way to comprehend and confront this complicated event.Materials and Methods: Male NMRI mice, weighing 20-25g, were used in this study.They were divided into six groups. The first group received 0.9% saline as the controlgroup and the other group was treated with morphine sulphate (30 mg/kg). LiCl (5 and 10mg/kg treatments) was separately performed in two other groups. The final two groupswere simultaneously treated with morphine sulphate (30 mg/kg) and LiCl (5 mg/kg) inone group and morphine sulphate (30 mg/kg) accompanied by LiCl (10 mg/kg) in theother group. All injections were performed intraperitoneally and once daily. After a fiveday wash-out, mice were decapitated and the brain regions which included the striatum,prefrontal cortex (PFC) and hippocampus were extracted. Using relative Real-Timepolymerase chain reaction (PCR), the expression levels of the long (D2L) and short (D2S)isoforms of the D2 receptor were investigated.Results: Morphine treatment leads to a significant increase (p

Details

Language :
English
ISSN :
22285814 and 22285806
Volume :
12
Issue :
3
Database :
OpenAIRE
Journal :
Cell Journal
Accession number :
edsair.dedup.wf.001..1eaa20b1b031409f67fca4355425ae41