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Microbes Are Associated with Host Innate Immune Response in Idiopathic Pulmonary Fibrosis
- Source :
- American journal of respiratory and critical care medicine, vol 196, iss 2
- Publication Year :
- 2017
- Publisher :
- eScholarship, University of California, 2017.
-
Abstract
- RationaleDifferences in the lung microbial community influence idiopathic pulmonary fibrosis (IPF) progression. Whether the lung microbiome influences IPF host defense remains unknown.ObjectivesTo explore the host immune response and microbial interaction in IPF as they relate to progression-free survival (PFS), fibroblast function, and leukocyte phenotypes.MethodsPaired microarray gene expression data derived from peripheral blood mononuclear cells as well as 16S ribosomal RNA sequencing data from bronchoalveolar lavage obtained as part of the COMET-IPF (Correlating Outcomes with Biochemical Markers to Estimate Time-Progression in Idiopathic Pulmonary Fibrosis) study were used to conduct association pathway analyses. The responsiveness of paired lung fibroblasts to Toll-like receptor 9 (TLR9) stimulation by CpG-oligodeoxynucleotide (CpG-ODN) was integrated into microbiome-gene expression association analyses for a subset of individuals. The relationship between associated pathways and circulating leukocyte phenotypes was explored by flow cytometry.Measurements and main resultsDown-regulation of immune response pathways, including nucleotide-binding oligomerization domain (NOD)-, Toll-, and RIG1-like receptor pathways, was associated with worse PFS. Ten of the 11 PFS-associated pathways correlated with microbial diversity and individual genus, with species accumulation curve richness as a hub. Higher species accumulation curve richness was significantly associated with inhibition of NODs and TLRs, whereas increased abundance of Streptococcus correlated with increased NOD-like receptor signaling. In a network analysis, expression of up-regulated signaling pathways was strongly associated with decreased abundance of operational taxonomic unit 1341 (OTU1341; Prevotella) among individuals with fibroblasts responsive to CpG-ODN stimulation. The expression of TLR signaling pathways was also linked to CpG-ODN responsive fibroblasts, OTU1341 (Prevotella), and Shannon index of microbial diversity in a network analysis. Lymphocytes expressing C-X-C chemokine receptor 3 CD8 significantly correlated with OTU1348 (Staphylococcus).ConclusionsThese findings suggest that host-microbiome interactions influence PFS and fibroblast responsiveness.
- Subjects :
- Male
bronchoalveolar lavage microbiome
CpG-oligodeoxynucleotide response
Respiratory System
Gene Expression
Down-Regulation
Bronchoalveolar Lavage
Autoimmune Disease
Medical and Health Sciences
Disease-Free Survival
Rare Diseases
Clinical Research
Genetics
Humans
Innate
2.1 Biological and endogenous factors
Aetiology
Lung
Life Below Water
Microbiota
COMET Investigators
Human Genome
Immunity
pattern recognition receptors
COMET-IPF Investigators
respiratory system
Middle Aged
Microarray Analysis
Flow Cytometry
Idiopathic Pulmonary Fibrosis
peripheral blood mononuclear cell transcriptome
host immune response and microbial interaction
Respiratory
Female
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- American journal of respiratory and critical care medicine, vol 196, iss 2
- Accession number :
- edsair.dedup.wf.001..1c608d4a31b7cea56b908506e109970a