a-C:H films produced by PECVD technique onto substrate of Ti6Al4V alloy: chemical and biological responses

Agradecimentos: The authors gratefully acknowledge the Federal University of São Carlos (UFSCar Sorocaba), Fatec Sorocaba, Federal University of Triângulo Mineiro (UFTM) for providing the laboratories facilities, as well as the National Counsel of Technological and Scientific Development (CNPq) grant 402142/2016-0, Coordination for the Improvement of Higher Education Personnel (CAPES) grant 88881.336769/2019-01-PVEX and FAPEMIG grant APQ-02276-18 for the financial support. The authors would also like to thank Professor Dra. Luciana Sgarbi Rossino and her master student Larissa Almeida for providing Ti6Al4V containing a-C:H samples for this study. The authors would like to thank the MSc. Guilherme de Lima Fernandes by the Raman spectroscopy analyzes. The authors would like to thank the Professor Graciela Ines Bolzon de Muniz by the analyzes Abstract: This work aims to investigate the chemical and biological responses of a-C:H films produced by PECVD technique onto substrate of Ti6Al4V alloy. The films produced were characterized morphologically and structurally by optical microscope (OM), SEM, AFM, EDX, FTIR Raman Spectroscopy (RS) and XPS. Afterwards, biocompatibility and cytotoxicity tests on osteoblastic cells and/or Human Peripheral Blood Mononuclear Cells (PBMCs) were performed on the Ti6Al4V alloy with coated and uncoated a-C:H film. The I-D/I-G ratio about 0.71 suggests a hydrogenated amorphous carbon film (a-C:H). The deconvoluted XPS spectra of C 1s peaks disclose the presence of three main components: Cl associated to C=C bonds in one sp(2) hybridization as well as C2 associated to sp(3) hybridization. The impedance results showed that a-C:H film acts as a protective barrier against the corrosion process. Regardless of the cell population, when the total apoptosis of osteoblastic cells or PBMCs was evaluated, only cells cultured on the alloy without a-C:H had significant induction of the apoptotic process. Moreover, only PBMCs cultured on the alloy without a-C:H had significant induction of inflammatory and anti-inflammatory cytokines that characterize immune activation CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIG Fechado