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Nuclear factor-kappa B in the liver of patients with chronic hepatitis C: decreased RelA expression is associated with enhanced fibrosis progression
- Source :
- Dadun. Depósito Académico Digital de la Universidad de Navarra, instname
- Publication Year :
- 2001
- Publisher :
- Wiley-Blackwell, 2001.
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Abstract
- The mechanisms of liver damage in chronic hepatitis C virus (HCV) infection are poorly understood. The transcription factor, nuclear factor-kappa B (NF-kappa B), regulates the expression of genes involved in apoptosis, inflammation, and antiviral response. It plays a protective role in several forms of liver damage. In this study, we analyzed NF-kappa B by gel mobility shift assay and immunohistochemistry in liver biopsies from HCV-infected patients, and we have determined the hepatic levels of the components of the NF-kappa B system by semiquantitative polymerase chain reaction (PCR). We found that NF-kappa B was activated in the liver of patients with chronic hepatitis C. Neither NF-kappa B activity nor the RNA levels of NF-kappa B subunits showed correlation with liver inflammatory activity, viral load, or HCV genotype. By contrast, hepatic mRNA values of RelA, the main element of active NF-kappa B, correlated inversely with apoptosis (r = -.68; P
- Subjects :
- Liver/metabolism
NF-kappa B/metabolism
Hepatitis C, Chronic/metabolism
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Dadun. Depósito Académico Digital de la Universidad de Navarra, instname
- Accession number :
- edsair.dedup.wf.001..15311b2f77a9b4b5b730f0f12227d074