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Widespread colonization of the lung by Tropheryma whipplei in HIV infection
- Source :
- American journal of respiratory and critical care medicine, vol 187, iss 10
- Publication Year :
- 2013
- Publisher :
- eScholarship, University of California, 2013.
-
Abstract
- RationaleLung infections caused by opportunistic or virulent pathogens are a principal cause of morbidity and mortality in HIV infection. It is unknown whether HIV infection leads to changes in basal lung microflora, which may contribute to chronic pulmonary complications that increasingly are being recognized in individuals infected with HIV.ObjectivesTo determine whether the immunodeficiency associated with HIV infection resulted in alteration of the lung microbiota.MethodsWe used 16S ribosomal RNA targeted pyrosequencing and shotgun metagenomic sequencing to analyze bacterial gene sequences in bronchoalveolar lavage (BAL) and mouths of 82 HIV-positive and 77 HIV-negative subjects.Measurements and main resultsSequences representing Tropheryma whipplei, the etiologic agent of Whipple's disease, were significantly more frequent in BAL of HIV-positive compared with HIV-negative individuals. T. whipplei dominated the community (>50% of sequence reads) in 11 HIV-positive subjects, but only 1 HIV-negative individual (13.4 versus 1.3%; P = 0.0018). In 30 HIV-positive individuals sampled longitudinally, antiretroviral therapy resulted in a significantly reduced relative abundance of T. whipplei in the lung. Shotgun metagenomic sequencing was performed on eight BAL samples dominated by T. whipplei 16S ribosomal RNA. Whole genome assembly of pooled reads showed that uncultured lung-derived T. whipplei had similar gene content to two isolates obtained from subjects with Whipple's disease.ConclusionsAsymptomatic subjects with HIV infection have unexpected colonization of the lung by T. whipplei, which is reduced by effective antiretroviral therapy and merits further study for a potential pathogenic role in chronic pulmonary complications of HIV infection.
- Subjects :
- Lung HIV Microbiome Project
Respiratory System
Tropheryma
microbiome
HIV Infections
Medical and Health Sciences
metagenome
Cohort Studies
Clinical Research
Genetics
Humans
bronchoalveolar lavage
2.2 Factors relating to the physical environment
2.1 Biological and endogenous factors
Longitudinal Studies
human
Aetiology
Lung
Human Genome
virus diseases
Infectious Diseases
Respiratory
HIV/AIDS
Infection
Whipple Disease
16S ribosomal RNA
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- American journal of respiratory and critical care medicine, vol 187, iss 10
- Accession number :
- edsair.dedup.wf.001..016d2a02ad0fac73c70f6107adedd205