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Calanoid copepod adminidstration improves yellow tail clownfish (Amphiprion clarkii) larviculture: biochemical and molecular implications
- Source :
- Aquaculture, Aquarium, Conservation & Legislation (Online) 2 (2009): 355–367., info:cnr-pdr/source/autori:Olivotto, I.; Avella, M.A., Buttino, I.; Cutignano, A.; Carnevali, O./titolo:Calanoid copepod adminidstration improves yellow tail clownfish (Amphiprion clarkii) larviculture: biochemical and molecular implications/doi:/rivista:Aquaculture, Aquarium, Conservation & Legislation (Online)/anno:2009/pagina_da:355/pagina_a:367/intervallo_pagine:355–367/volume:2
- Publication Year :
- 2009
- Publisher :
- Bioflux, Cluj-Napoca, Romania, 2009.
-
Abstract
- In this study we evaluated the effects of a standard rotifers/Artemia(control) diet and a copepod (Centropages typicus) based diet duringAmphiprion clarkii larviculture. On day 11 post hatch, larvae fed C. typicus showed better survival and growth compared to those fed a standard rotifer/ Artemia diet (87±2 vs 41±2%;6.8± 0.2mm vs 6.1± 0.2mm; 5.9±0.3mg vs 4.5± 0.3 mg,respectively). Further molecular analysis have been carried out on 5 and 11 days post hatch larval samples to clarify the efficiency of a copepod based diet respect to control. In particular, the peroxisome proliferator activated receptors (PPAR-?,-?) gene expression, which are involved in several biological processes such as lipid metabolism, the retinoic acid receptor ? (RAR ? ), involved in vitamin A metabolism and the heat shock protein 70 (HSP70), a biomarkers of cellular stress, were a nalyzed by real -time PCR during the larval development. The results obtained are related to fatty acid composition of live preys used. The superiority of a Centropages typicus copepod diet respect to a standard rotifers-Artemia one has been demonstrated; these results are essential to improve captive production of A. clarkii through a closed system and in turn to preserve natural stocks.
- Subjects :
- copepods
clownfish
Artemia
fatty acids
rotifers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Aquaculture, Aquarium, Conservation & Legislation (Online) 2 (2009): 355–367., info:cnr-pdr/source/autori:Olivotto, I.; Avella, M.A., Buttino, I.; Cutignano, A.; Carnevali, O./titolo:Calanoid copepod adminidstration improves yellow tail clownfish (Amphiprion clarkii) larviculture: biochemical and molecular implications/doi:/rivista:Aquaculture, Aquarium, Conservation & Legislation (Online)/anno:2009/pagina_da:355/pagina_a:367/intervallo_pagine:355–367/volume:2
- Accession number :
- edsair.cnr...........dadb49229e5969cd3487ccd4900cdc5e