Immune responses gene expression in broiler chickens fed mealworm meal

Introduction. Over the last decade several studies were done to assess the nutritional composition and effects of partial substitution of traditional protein sources with insect meal in animal health. The aim of this study was to assess the effect of dietary Tenebrio molitor (TM) meal supplementation on immune and cellular responses to external stimuli of intestinal mucosa and liver in poultry. Animals, material and methods. One-day old broiler chicks (Ross 708) were raised in 10 pens (8 birds/pen), and were divided in 2 groups. Two isonitrogenous and isoenergetic feeding plans were used (12.9 MJ ME/kg and 23.5% CP from day 1 until day 12; 13.3 MJ ME/kg and 21.3% CP from day 12 until day 25; and 13.5 MJ ME/kg and 19.6% CP from day 25 until slaughter) containing either 0% (CT) or 15% (TM) mealworm meal (52.4% CP, 28.0% EE and 8.8% ash as fed). Two broilers/pen were slaughtered at 53 days of age. Liver, jejunum, and caecum samples were collected and stored in RNA Later solution (Ambion) at -80°C pending analysis. Pooled samples of each treatment were analyzed for RNA-seq using Illumina NGS analyzer. Differential expression of genes (DEG) was carried out using Cuffdiff test. Fisher test corrected with Bonferroni procedure [1] was used for statistical analysis of RNA sequencing data. Significant differences were considered for p