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Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants

Authors :
Silvestri, Fausto)[ 1,2,3 ]
Vitiello, V [ 2,3 ]
Del Prete, F [ 2,3 ]
Fabbrocini, A [ 4 ]
Ferreira, JF [ 5 ]
Sansone, G [ 2,3 ]
Source :
Aquaculture research, 47 (2016): 624–631., info:cnr-pdr/source/autori:Silvestri, Fausto)[ 1,2,3 ] ; Vitiello, V [ 2,3 ] ; Del Prete, F [ 2,3 ] ; Fabbrocini, A [ 4 ] ; Ferreira, JF [ 5 ] ; Sansone, G [ 2,3 ]/titolo:Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants/doi:/rivista:Aquaculture research (Print)/anno:2016/pagina_da:624/pagina_a:631/intervallo_pagine:624–631/volume:47
Publication Year :
2016
Publisher :
Blackwell Scientific, Oxford , Regno Unito, 2016.

Abstract

Cryopreservation is a valuable tool for aquaculture as it provides continuous seed production, regardless of the spawning season of the brood stock. The selection of a suitable cryoprotectant with low toxicity and high water solubility is important to avoid membrane injuries and intracellular ice crystallization. This study has been aimed at the assessment of the toxic effects of two usually applied cryoprotectants, 1-2 propylene glycol (PG) and methanol (MetOH), on spermatozoa of the of lion-paw scallop Nodipecten nodosus, by evaluating the sperm motility and the development of D larvae after fertilization procedure. Sperm was exposed at room temperature (22 degrees C) for 10, 20 and 30min to different concentration ranges of two cryoprotectants. Regarding the sperm motility, PG5%, PG7%, MetOH4% and MetOH6% did not show differences compared to control (semen incubated in seawater) (P

Subjects

Subjects :
lion-paw scallop
1-2 propylene glycol
methanol
cryopreservation

Details

Language :
English
Database :
OpenAIRE
Journal :
Aquaculture research, 47 (2016): 624–631., info:cnr-pdr/source/autori:Silvestri, Fausto)[ 1,2,3 ] ; Vitiello, V [ 2,3 ] ; Del Prete, F [ 2,3 ] ; Fabbrocini, A [ 4 ] ; Ferreira, JF [ 5 ] ; Sansone, G [ 2,3 ]/titolo:Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants/doi:/rivista:Aquaculture research (Print)/anno:2016/pagina_da:624/pagina_a:631/intervallo_pagine:624–631/volume:47
Accession number :
edsair.cnr...........2fe900b7e71d550a3587f9acd9a510b2

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