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N- and O-linked glycosylation site profiling ot the human basic salivary proline-rich protein 3M

Authors :
B. Manconi
T. Cabras
M. Sanna
V. Piras
B. Liori
E. Pisano
F. Iavarone
F. Vincenzoni
M. Cordaro
G. Faa
M. Castagnola
I. Messana
Source :
Journal of proteomics (Online) (2016)., info:cnr-pdr/source/autori:B. Manconi, T. Cabras, M. Sanna, V. Piras, B. Liori, E. Pisano, F. Iavarone, F. Vincenzoni, M. Cordaro, G. Faa, M. Castagnola, I. Messana/titolo:N-and O-linked glycosylation site profiling ot the human basic salivary proline-rich protein 3M/doi:/rivista:Journal of proteomics (Online)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume
Publication Year :
2016
Publisher :
Elsevier, Amsterdam , Paesi Bassi, 2016.

Abstract

The acid-insoluble salivary proteome obtained by addition of TFA to whole human saliva from adults, preterm and at-term newborns has been analysed by 2-DE in order to evidence differences among the three groups, and integrate data previously obtained on the acid-soluble fraction. 2-DE spots differentially expressed among the three groups were submitted to in-gel tryptic digestion and the peptide mixtures analysed by high resolution HPLC-ESI-MS/MS. By this strategy, we identified 3 over-expressed proteins in at-term newborns with respect to preterm newborns and adults (BPI fold-containing family A member 1, annexin A1, and keratin type 1 cytoskeletal 13), and several over-expressed proteins in adults (fatty acid-binding protein, S100 A6, S100 A7, S100 A9, prolactin-inducible protein, Ig kappa chain, cystatin SN, cystatin S/SA and ?-amylase 1). Four spots, already detected but not characterized by other authors in human saliva 2-DE, were attributed to different protein species of S100 A9 (long-type and long-type monophosphorylated, short-type and short-type monophosphorylated) by MS/MS analysis of tryptic peptides and sequential staining of 2-DE gels with Pro-Q Diamond, for specific detection of phosphoproteins, and total protein SYPRO Ruby stain.

Details

Language :
English
Database :
OpenAIRE
Journal :
Journal of proteomics (Online) (2016)., info:cnr-pdr/source/autori:B. Manconi, T. Cabras, M. Sanna, V. Piras, B. Liori, E. Pisano, F. Iavarone, F. Vincenzoni, M. Cordaro, G. Faa, M. Castagnola, I. Messana/titolo:N-and O-linked glycosylation site profiling ot the human basic salivary proline-rich protein 3M/doi:/rivista:Journal of proteomics (Online)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume
Accession number :
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