Back to Search Start Over

Massive transcriptome sequencing in sole

Massive transcriptome sequencing in sole

Authors :
Manchado M., Infante C., Ponce M., Planas J. V., Cañavate J. P.
Source :
Workshop The Cultivation of Soles V, Servifapa. Repositorio Institucional del IFAPA, instname
Publication Year :
2011
Publisher :
Workshop The Cultivation of Soles V, 2011.

Abstract

The rapid development of Next Generation Sequencing (NGS) techniques has transformed the way genomic research and characterization of genomes is done today. They can be applied to a wide variety of biological questions, from the sequencing of complete eukaryotic and prokaryotic genomes and transcriptomes, genome-scale analysis of DNA–protein interactions to metapopulation studies. Ultra-high throughput sequencing of the transcriptome (RNA-seq) has become a powerful and attractive alternative technology for gene discovery, transcript quantification and marker discovery. In this presentation, we focus on the use of NGS in sole transcriptomics. Particularly, we have performed two 454 runs for transcriptome analysis in Senegalese sole. In the first run, we tried to evaluate the defence mechanisms against pathogens throughout a better knowledge of the transcriptome of five immunostimulated organs (a pool of Head kidney, spleen, thymus, brain, gills). Presentación oral en Workshop The Cultivation of Soles V. 5-7 Abril, 2011. Faro (Portugal).

Details

Database :
OpenAIRE
Journal :
Workshop The Cultivation of Soles V, Servifapa. Repositorio Institucional del IFAPA, instname
Accession number :
edsair.RECOLECTA.....f1a128cf5506e4a3444be635cee17a70