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Isolation and identification of hevein as a major IgE-binding polypeptide in Hevea latex
- Source :
- The Journal of Allergy and Clinical Immunology; March 1997, Vol. 99 Issue: 3 p402-406, 5p
- Publication Year :
- 1997
-
Abstract
- Background: Polypeptides in Hevea latex are known as the major cause of latex type I sensitivities. So far, only a few of them have been characterized. Methods: Proteins with a molecular weight lower than 10 kd in fresh Hevea latex were separated by ultrafiltration and further characterized by liquid chromatography on-line-coupled electrospray mass spectrometry. Hevein in this fraction was then purified by preparative reverse-phase high-performance liquid chromatography and characterized by matrix-assisted laser desorption ionization mass spectrometry and protein sequencing. Skin prick tests, enzyme-linked allergosorbent tests, and inhibition immunoblotting were performed to show the allergenicity of the purified hevein. Results: Hevein, a 4.7 kd polypeptide, is the predominant component in the fraction with latex proteins of smaller than 10 kd. Specific IgE antibodies to hevein were detected by enzyme-linked allergosorbent test in 48 of 64 (75%) sera from health care workers allergic to latex and in three of 11 (27%) sera from patients with spina bifida and hypersensitivity reactions to latex. Inhibition immunoblotting demonstrated that the preincubation of 14 sera and a serum pool from patients allergic to latex with purified hevein completely inhibited IgE binding to the 20 kd protein, which has been recently reported to be a major allergen in latex (prohevein). Skin prick testing showed a positive reaction to hevein in 17 of 21 (81%) patients with latex allergy. Conclusions: The results clearly demonstrate that hevein is an important latex allergen, and the IgE-binding capacity of prohevein in latex is mostly attributed to hevein, the N-terminal domain of prohevein. ( J Allergy Clin Immunol 1997;99:402-9.)
Details
- Language :
- English
- ISSN :
- 00916749 and 10976825
- Volume :
- 99
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- The Journal of Allergy and Clinical Immunology
- Publication Type :
- Periodical
- Accession number :
- ejs9930036
- Full Text :
- https://doi.org/10.1016/S0091-6749(97)70059-9