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Regulation of leukocyte adhesion molecules by leukocyte/biomaterial-conditioned media: A study with calcium-phosphate-coated and non-coated NiTi-shape memory alloys
- Source :
- Materialwissenschaft und Werkstofftechnik; June 2006, Vol. 37 Issue: 6 p558-562, 5p
- Publication Year :
- 2006
-
Abstract
- To evaluate the role of soluble mediators released after interaction of leukocytes with biomaterials on cells of the implant microenvironment, the surface expression of adhesion molecules (CD11b, CD62L, CD66b, ICAM-1) on freshly isolated whole blood leukocytes (WBL) was analyzed by flow cytometry after incubation in leukocyte/nickel-titanium shape-memory alloy (NiTi-SMA)-conditioned media. NiTi-SMA samples were either coated with calcium phosphate by dipping in oversaturated calcium phosphate solution (CaP-coating) or were non-coated. Peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) were isolated and seeded on both samples to generate respective conditioned media. In comparison with conditioned media obtained by non-coated NiTi-SMA, the expression of CD11b, CD66b, and ICAM-1 on WBL was upregulated by conditioned media obtained from CaP-coated samples. In contrast, the expression of CD62L on WBL was decreased after incubation in conditioned medium obtained from CaP-coated NiTi-samples compared to non-coated NiTi. These data demonstrate the possible influence of released mediators elicited by leukocyte-biomaterial interactions on cells of the implant microenvironment.
Details
- Language :
- English
- ISSN :
- 09335137 and 15214052
- Volume :
- 37
- Issue :
- 6
- Database :
- Supplemental Index
- Journal :
- Materialwissenschaft und Werkstofftechnik
- Publication Type :
- Periodical
- Accession number :
- ejs9318930
- Full Text :
- https://doi.org/10.1002/mawe.200600037