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Molecular Cloning and Characterization of a Vacuolar H+-pyrophos-phatase Gene, SsVP, from the Halophyte Suaeda salsaand its Overexpression Increases Salt and Drought Tolerance of Arabidopsis
- Source :
- Plant Molecular Biology; January 2006, Vol. 60 Issue: 1 p41-50, 10p
- Publication Year :
- 2006
-
Abstract
- The chenopodiaceae Suaeda salsaL. is a leaf succulent euhalophyte. Shoots of the S. salsaare larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsadoes not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na+in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H+-PPase) from S. salsa. The SsVPcDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVPwas induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsisoverexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVPcan increase salt and drought tolerance of transgenic Arabidopsis.The chenopodiaceae Suaeda salsaL. is a leaf succulent euhalophyte. Shoots of the S. salsaare larger and more succulent when grown in highly saline environments. This increased growth and water uptake has been correlated with a large and specific cellular accumulation of sodium. S. salsadoes not have salt glands or salt bladders on its leaves. Thus, this plant must compartmentalize the toxic Na+in the vacuoles. The ability to compartmentalize sodium may result from a stimulation of the proton pumps that provide the driving force for increased sodium transport into the vacuole. In this work, we isolated the cDNA of the vacuolar membrane proton-translocating inorganic pyrophosphatase (H+-PPase) from S. salsa. The SsVPcDNA contains an uninterrupted open reading frame of 2292 bp, coding for a polypeptide of 764 amino acids. Northern blotting analysis showed that SsVPwas induced in salinity treated leaves. The activities of both the V-ATPase and the V-PPase in Arabidopsisoverexpressing SsVP-2 is higher markedly than in wild-type plant under 200 mM NaCl and drought stresses. The Overexpression of SsVPcan increase salt and drought tolerance of transgenic Arabidopsis.
Details
- Language :
- English
- ISSN :
- 01674412 and 15735028
- Volume :
- 60
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Plant Molecular Biology
- Publication Type :
- Periodical
- Accession number :
- ejs8478851
- Full Text :
- https://doi.org/10.1007/s11103-005-2417-6