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CTX-M-15 extended-spectrum β-lactamase from Nigerian Klebsiella pneumoniae
- Source :
- Journal of Antimicrobial Chemotherapy (JAC); January 2006, Vol. 57 Issue: 1 p24-30, 7p
- Publication Year :
- 2006
-
Abstract
- Objectives: In this study, extended-spectrum β-lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria.Methods: The β-lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the blaCTX-M genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNA–DNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants.Results: All 30 isolates produced at least one β-lactamase. Seventeen of the isolates were resistant to cefotaxime, and had ≥100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have blaCTX-M genes that were associated with large plasmids (≥58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 β-lactamases. The ISEcp1 element was located upstream of blaCTX-M-15 in the same position as previously described. In addition, 23 of the isolates produced TEM β-lactamases, 27 produced SHV β-lactamases and four produced AmpC β-lactamases.Conclusions: Thirty K. pneumoniae produced multiple β-lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa.
Details
- Language :
- English
- ISSN :
- 03057453 and 14602091
- Volume :
- 57
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Periodical
- Accession number :
- ejs8229188
- Full Text :
- https://doi.org/10.1093/jac/dki429