Back to Search
Start Over
Immunogenicity and Protective Efficacy of Recombinant Human T-Cell Leukemia/Lymphoma Virus Type 1 NYVAC and Naked DNA Vaccine Candidates in Squirrel Monkeys (Saimiri sciureus)
- Source :
- The Journal of Virology; July 2001, Vol. 75 Issue: 13 p5939-5948, 10p
- Publication Year :
- 2001
-
Abstract
- ABSTRACTWe assessed the immunogenicities and efficacies of two highly attenuated vaccinia virus-derived NYVAC vaccine candidates encoding the human T-cell leukemia/lymphoma virus type 1 (HTLV-1) envgene or both the envand gaggenes in prime-boost pilot regimens in combination with naked DNA expressing the HTLV-1 envelope. Three inoculations of NYVAC HTLV-1 envat 0, 1, and 3 months followed by a single inoculation of DNAenvat 9 months protected against intravenous challenge with HTLV-1-infected cells in one of three immunized squirrel monkeys. Furthermore, humoral and cell-mediated immune responses against HTLV-1 Env could be detected in this protected animal. However, priming the animal with a single dose of envDNA, followed by immunization with the NYVAC HTLV-1 gagandenvvaccine at 6, 7, and 8 months, protected all three animals against challenge with HTLV-1-infected cells. With this protocol, antibodies against HTLV-1 Env and cell-mediated responses against Env and Gag could also be detected in the protected animals. Although the relative superiority of a DNA prime-NYVAC boost regimen over addition of the Gag component as an immunogen cannot be assessed directly, our findings nevertheless show that an HTLV-1 vaccine approach is feasible and deserves further study.
Details
- Language :
- English
- ISSN :
- 0022538X and 10985514
- Volume :
- 75
- Issue :
- 13
- Database :
- Supplemental Index
- Journal :
- The Journal of Virology
- Publication Type :
- Periodical
- Accession number :
- ejs7948679
- Full Text :
- https://doi.org/10.1128/JVI.75.13.5939-5948.2001