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Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5.

Authors :
Shalekoff, S
Tiemessen, C T
Source :
Clinical and Vaccine Immunology (formerly CDLI); March 2001, Vol. 8 Issue: 2 p432-6, 5p
Publication Year :
2001

Abstract

Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.

Details

Language :
English
ISSN :
15566811 and 1556679X
Volume :
8
Issue :
2
Database :
Supplemental Index
Journal :
Clinical and Vaccine Immunology (formerly CDLI)
Publication Type :
Periodical
Accession number :
ejs7840618
Full Text :
https://doi.org/10.1128/CDLI.8.2.432-436.2001