Characterization of Pepsinogen C as a Potential Biomarker for Gastric Cancer Using a Histo-Proteomic Approach

Authors :: Melle, C.
Ernst, G.
Schimmel, B.
Bleul, A.
Kaufmann, R.
Hommann, M.
Richter, K. K.
Daffner, W.
Settmacher, U.
Claussen, U.
Eggeling, F. von
Source :: Journal of Proteome Research; October 2005, Vol. 4 Issue: 5 p1799-1804, 6p
Publication Year :: 2005
Abstract: We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 × 10<SUP>-6</SUP>) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry. Keywords: gastric cancer • proteomics • biomarker • ProteinChip technology • SELDI • microdissection • immunohistochemistry

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