Artificial Imitation of Glutathione Peroxidase with 6-Selenium-Bridged β-Cyclodextrin

On the basis of cyclodextrin, 6-selenium bridged β-cyclodextrin (6-β-CD-Se-Se-β-CD, known as 6-SeCD) was synthesized by the selective tosylation of β-cyclodextrin and nucleophilic displacement by sodium hydroselenide to imitate glutathione peroxidase (GPX). The GPX activity of diselenide 6-SeCD is 4.3 times that of PZ51. The structure of the mimic 6-SeCD was characterized by means of laser mass spectroscopy, elemental analysis, IR and1H NMR. The selenium content and its valence in 6-SeCD were determined by means of X-ray photoelectron spectra. Kinetics of the mimic showed that its enzymatic behavior was similar to that of native GPX.