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Laser-Induced Optoacoustic Spectroscopy (LIOAS) of Proteins: Spectrum of Bovine Serum Albumin in the 532–670 nm Region

Authors :
Roveri, Oscar A.
Bilmes, Gabriel M.
Heihoff, Klaus
Braslavsky, Silvia E.
Source :
Applied Spectroscopy; December 1990, Vol. 44 Issue: 10 p1706-1710, 5p
Publication Year :
1990

Abstract

Bovine serum albumin, as well as other proteins, produces optoacoustic signals when excited with a laser pulse at wavelengths from 532 to 670 nm, a region where these proteins have no reported absorption. Signals from filtered solutions (obtained at low fluences) exhibited a linear dependence on laser energy and on protein concentration. Hence, they are attributed to radiationless deactivation following weak absorption in the visible region. Molar absorption coefficients calculated for different proteins showed a similar wavelength dependence, albeit with different absolute values—differences that could not be simply correlated to the content of known protein chromophores, such as tryptophan, tyrosine, and peptide bond. Moreover, at 532 nm, such chromophores produced optoacoustic signals only slightly higher than those from the buffer. On the other hand, a synthetic copolymer of tryptophan and lysine produced signals of a magnitude comparable to those from the proteins. Therefore, the weak absorption detected by laser-induced optoacoustics should be attributed to a not-yet-well-established feature of the structure of the protein, probably related to charge transfer interactions between different amino acids. Regardless of their origin, these signals must be taken into account when chromophoric prosthetic groups are studied with the use of laser-induced optoacoustic spectroscopy.

Details

Language :
English
ISSN :
00037028
Volume :
44
Issue :
10
Database :
Supplemental Index
Journal :
Applied Spectroscopy
Publication Type :
Periodical
Accession number :
ejs7407612
Full Text :
https://doi.org/10.1366/0003702904417616