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Nuclear Import of Spo12p, a Protein Essential for Meiosis*
- Source :
- Journal of Biological Chemistry; May 2001, Vol. 276 Issue: 21 p17712-17717, 6p
- Publication Year :
- 2001
-
Abstract
- In Saccharomyces cerevisiae, Spo12p is involved in mitosis and is essential for meiosis. We found that Spo12p is imported into the nucleus by the karyopherin Kap121p. A complex containing Spo12p and Kap121p was isolated from cytosol and was also reconstituted with recombinant proteins, indicating that this interaction is direct. Spo12p was mislocalized to the cytosol inpse1–1, a temperature-sensitive strain harboring a mutation of Kap121p, at the permissive temperature, confirming an essential role for Kap121p in Spo12p import. Spo12p was also mislocalized in a pse1–1/pse1–1homozygous strain, suggesting it is imported via the same pathway in diploid cells. Furthermore, we found that pse1–1/pse1–1shows a sporulation defect similar to that ofspo12Δ/spo12Δ.In addition, we have characterized the Spo12p nuclear localization signal, mapped it to residues 76–130, and identified residues within this region that are important for nuclear localization signal function.
Details
- Language :
- English
- ISSN :
- 00219258 and 1083351X
- Volume :
- 276
- Issue :
- 21
- Database :
- Supplemental Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs7170861
- Full Text :
- https://doi.org/10.1074/jbc.M010760200