Back to Search
Start Over
Replica multichannel polymer chips with a network of sacrificial channels sealed by adhesive printing method
- Source :
- Lab on a Chip - Miniaturisation for Chemistry and Biology; 2005, Vol. 5 Issue: 4 p472-478, 7p
- Publication Year :
- 2005
-
Abstract
- Replica microchips for capillary array electrophoresis containing 10 separation channels 50 µm width, 50 µm depth and 100 µm pitch and a network of sacrificial channels 100 µm width and 50 µm depth were successfully fabricated on a polymethyl methacrylate PMMA substrate by injection molding. The strategy involved development of moving mask deep X-ray lithography to fabricate an array of channels with inclined channel sidewalls. A slight inclination of channel sidewalls, which can not be fabricated by conventional deep X-ray lithography, is highly required to ensure the release of replicated polymer chips from a mold. Moreover, the sealing of molded PMMA multichannel chips with a PMMA cover film was achieved by a novel bonding technique involving adhesive printing and a network of sacrificial channels. An adhesive printing process enables us to precisely control the thickness of an adhesive layer, and a network of sacrificial channels makes it possible to remove air bubbles and an excess adhesive, which are crucial to achieving perfect sealing of replica PMMA chips with well-defined channel and injection structures. A CCD camera equipped with an image intensifier was used to simultaneously monitor electrophoretic separations in ten micro-channels with laser-induced fluorescence detection. High-speed and high-throughput separations of a 100 bp DNA ladder and X174 HaeIII DNA restriction fragments have been demonstrated using a 10-channel PMMA chip. The current work establishes the feasibility of mass production of PMMA multichannel chips at a cost-effective basis.
Details
- Language :
- English
- ISSN :
- 14730197 and 14730189
- Volume :
- 5
- Issue :
- 4
- Database :
- Supplemental Index
- Journal :
- Lab on a Chip - Miniaturisation for Chemistry and Biology
- Publication Type :
- Periodical
- Accession number :
- ejs6954657
- Full Text :
- https://doi.org/10.1039/b417398h