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Electrochemical Detection of Gentamicin Using a Reduced Graphene Oxide-Yttria Oxide (rGO@Y2O3) Nanocomposite-Based Biosensor

Authors :
Himanshu, Jayendra Kumar
Verma, Damini
Lakshmi, G. B. V. S.
Singh, Akhilesh Kumar
Solanki, Pratima R.
Source :
Journal of the Electrochemical Society; December 2024, Vol. 171 Issue: 12 p127510-127510, 1p
Publication Year :
2024

Abstract

The control of antibiotic resistance is at an alarming stage, making the detection of antibiotics as utmost importantance. A sono-chemically-synthesized reduced-graphene oxide-yttrium oxide nanocomposite (rGO@Y2O3NC) was used to develop an effective electrochemical immunosensing platform for gentamicin detection. The rGO@Y2O3NC was characterized using different techniques, providing insights into its structural and functional properties. The detection was carried out on a carbon screen printed electrode modified with rGO@Y2O3NC using differential pulse voltammetry technique. The rGO@Y2O3NC helped to enhance the electrochemical activity and the functional groups present on rGO helped in efficient electrostatic immobilization of the antibody. Protein kinase A triggered the immunologic interaction and hence helped in achieving higher sensitivity. The developed immunosensor exhibited an impressive limit of detection of 0.457 pM, with a wide range of detection from 1 pM to 100 μM with a sensitivity of 7.03 μA pM−1cm−2, representing a substantial enhancement over current technologies. Furthermore, the immunosensor demonstrated practical applicability by effective performance in spiked milk and tap water samples. This highlights its potential for real-world applications in monitoring antibiotic use and ensuring water and food quality control according to regulations.

Details

Language :
English
ISSN :
00134651 and 19457111
Volume :
171
Issue :
12
Database :
Supplemental Index
Journal :
Journal of the Electrochemical Society
Publication Type :
Periodical
Accession number :
ejs68396265
Full Text :
https://doi.org/10.1149/1945-7111/ad94a9