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Assembly of Recombinant Proteins into β-Sheet Fibrillating Peptide-Driven Supramolecular Hydrogels for Enhanced Diabetic Wound Healing

Authors :
Guo, Zhao
Liu, Xing
Xia, Yan
Wang, Jie
Li, Jiaqi
Wang, Liping
Li, Yimiao
Jia, Shuang
Sun, Yinan
Feng, Jian
Huang, Jingxia
Dong, Yuxin
Wang, Liyao
Li, Xinyu
Source :
ACS Biomaterials Science & Engineering; January 2025, Vol. 11 Issue: 1 p228-238, 11p
Publication Year :
2025

Abstract

Supramolecular hydrogels offer a noncovalent binding platform that preserves the bioactivity of structural molecules while enhancing their stability, particularly in the context of diabetic wound repair. In this study, we developed protein–peptide-based supramolecular hydrogels by assembling β-sheet fibrillizing peptides (designated Q11) with β-tail fused recombinant proteins. The Q11 peptides have the ability to drive the gradated assembly of N- or C-terminal β-sheet structure (β-tail) fused recombinant proteins. We first investigated the assembly properties of Q11 and assessed its stability under varying pH and temperature conditions by combining Q11 with two β-tail fused fluorescent proteins. The results showed that Q11 enhanced the tolerance of the fluorescent proteins to changes in pH and temperature. Building upon these findings, we designed collagen-like proteins and Sonic Hedgehog-fused recombinant proteins (CLP-Shh) that could be assembled with Q11 to form peptide–protein supramolecular hydrogels. These hydrogels demonstrated the ability to improve cell viability and migration and upregulate key markers of cell growth. Further in vivo studies revealed that the Q11-driven supramolecular hydrogel effectively enhances diabetic wound healing and epidermal regeneration by promoting the expression of epidermal-related proteins and immune factors. This study highlights the potential of supramolecular hydrogels for clinical applications and their promise in the development of biofunctional hydrogels for therapeutic use.

Details

Language :
English
ISSN :
23739878
Volume :
11
Issue :
1
Database :
Supplemental Index
Journal :
ACS Biomaterials Science & Engineering
Publication Type :
Periodical
Accession number :
ejs68230959
Full Text :
https://doi.org/10.1021/acsbiomaterials.4c01723