Utility of icobrain for brain volumetry in multiple sclerosis clinical practice

•In this real-world clinical MS setting, using icobrain for analysis of brain atrophy and our quality control criteria, we found comparable variance on brain volume changes for same-scanner and different-scanner MRI pairs•A higher number of different-scanner MRI pairs did not pass quality control and had poorer alignment similarity•Applying strict selection criteria for volumetric analyses in real-world settings, and longitudinal imaging within scanners, remain imperative to reduce brain atrophy variability