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Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing
- Source :
- Genome Biology; October 2003, Vol. 4 Issue: 10
- Publication Year :
- 2003
-
Abstract
- Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing.
Details
- Language :
- English
- ISSN :
- 14747596 and 1474760X
- Volume :
- 4
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Genome Biology
- Publication Type :
- Periodical
- Accession number :
- ejs6784266
- Full Text :
- https://doi.org/10.1186/gb-2003-4-10-r66