Regulatory sequences in the promoter of the Dictyostelium Actin 6 gene.

The promoter region of the developmentally regulated Actin 6 gene of Dictyostelium has been dissected by a series of deletions. Functional analysis of the deletions in Dictyostelium transformants revealed two short regulatory sequences: a positive upstream element (PUE) between ‐599 and ‐572 which increases transcription by a factor of 10 but does not affect the developmental pattern of expression and an upstream activator sequence (UAS) between ‐249 and ‐215 which is essential for transcription and proper developmental regulation. The UAS partially coincides with a conserved sequence with dyad symmetry found upstream of several Dictyostelium actin genes (Romans and Firtel, 1985a).