Effect of acute starvation on plasma fibronectin response to sepsis

There is evidence that undernutrition may contribute to the reduction in plasma fibronectin concentration and the depression of the reticuloendothelial (RE) system associated with severe sepsis. We have investigated the effects of fasting, surgical trauma and sepsis on plasma fibronectin concentrations and RE function. In experiment 1, plasma fibronectin was measured in rabbits (n= 14) before and 48 h after fasting. In experiment 2, sepsis was induced by devascularization of the appendix in animals on a normal diet (sepsis group, n= 7) and in fas ted animals (fasting and sepsis group, n= 7). A third group of animals underwent only a laparotomy (laparotomy only group, n= 7). Plasma fibronectin concentrations and the blood clearance and organ distribution of 99mtechnetium tin colloid (TTC) were measured 24h after operation. Compared with pooled reference plasma, fasting in experiment 1 resulted in a reduction in mean(s.e.m.) plasma fibronectin concentration from 98(1·5) per cent to 86(3·7) per cent (T= 2, P< 0·005). Mean(s.e.m.) plasma fibronectin concentration was raised in the sepsis group to 117(4·6)per cent, compared with 97(2·5)per cent in the laparotomy only group (U= 5, P< 0·02), but there was no such increase in the fasting and sepsis group. There was a delay in the blood clearance and reduced hepatic uptake of TTC in both sepsis groups. The dissociation between fibronectin concentrations and RE function in animal models of sepsis casts doubt on the importance of fibronectin in RE function.