Responses of soil microbial metabolism, function and soil quality to long-term addition of organic materials with different carbon sources

Ryegrass green manure (RG) and biochar (BC) induced the simplification of microbial networks and an increase in fungi’s random assembly processes.RG promoted denitrification and carbon degradation functional genes, while BC enhanced nitrogen fixation genes.RG accelerated soil energy flow by promoting M00307 and M00061.In RG-treated soil, the main metabolites were Carbohydrates, while in BC-treated soil, the main metabolites were Acids and Amines. Both treatments reduced soil pollutant bisphenol A.