Back to Search
Start Over
Protein Kinase A Activity And Protein Phosphorylation During The Mouse Sperm Acrosomal Reaction
- Source :
- Systems Biology in Reproductive Medicine; 2005, Vol. 51 Issue: 1 p55-64, 10p
- Publication Year :
- 2005
-
Abstract
- Using two-dimensional gel electrophoresis, changes in protein phosphorylation caused by cyclic nucleotide-dependent protein kinases were analyzed with or without exposure to a protein kinase inhibitor, H-8, during the mouse sperm acrosomal reaction. The acrosomal reaction, induced by the treatment of sperm with dibutyryl cyclic AMP or dibutyryl cyclic GMP, was inhibited by H-8. The activities of cyclic AMP-dependent protein kinase (PKA) and cyclic GMP-dependent protein kinase induced by the sperm extract were also inhibited by H-8. When endogenous PKA in sperm was activated by the addition of cyclic AMP, a 45-kDa protein spot identified by electrophoresis indicated the occurrence of phosphorylation in vivo. Furthermore, the enhanced phosphorylation of the 45-kDa protein spot was inhibited by H-8. These results suggest that the PKA-catalyzed phosphorylation of the 45-kDa protein may be involved in the regulation of the mouse sperm acrosomal reaction.
Details
- Language :
- English
- ISSN :
- 19396368 and 19396376
- Volume :
- 51
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Systems Biology in Reproductive Medicine
- Publication Type :
- Periodical
- Accession number :
- ejs6744137
- Full Text :
- https://doi.org/10.1080/014850190512743