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Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging
- Source :
- Nano Letters; October 2024, Vol. 24 Issue: 39 p12125-12132, 8p
- Publication Year :
- 2024
-
Abstract
- Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of ∼100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
Details
- Language :
- English
- ISSN :
- 15306984 and 15306992
- Volume :
- 24
- Issue :
- 39
- Database :
- Supplemental Index
- Journal :
- Nano Letters
- Publication Type :
- Periodical
- Accession number :
- ejs67432369
- Full Text :
- https://doi.org/10.1021/acs.nanolett.4c02452