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The mitogenic effect of platelet-derived growth factor in human hepatic stellate cells requires calcium influx
- Source :
- American Journal of Physiology - Cell Physiology; November 1995, Vol. 269 Issue: 5 pC1133-C1139, 7p
- Publication Year :
- 1995
-
Abstract
- Platelet-derived growth factor (PDGF) is a key mitogen for hepatic stellate cells (HSC) and has been shown to be implicated in liver tissue repair and fibrogenesis. In this study the relationship between PDGF-induced intracellular Ca2+ concentration ([Ca2+]i) increase and mitogenesis in cultured human HSC was evaluated. In high-density cell cultures (80-90% subconfluence), PDGF induced a significant increase in [Ca2+]i, characterized by a short-lasting peak phase, which was followed by a long-lasting plateau phase. The plateau phase was abolished in the absence of extracellular Ca2+. However, in low-density cell cultures (30-40% subconfluence), the plateau phase was absent or markedly less pronounced. In parallel sets of experiments, PDGF was significantly less effective in inducing mitogenesis in low-density cell cultures than in high-density cell cultures and was totally ineffective in the absence of extracellular Ca2+. These results suggest that 1) spatial and time dynamics of PDGF-induced [Ca2+]i increase are dependent on cell density and 2) PDGF-induced mitogenesis requires extracellular Ca2+ influx.
Details
- Language :
- English
- ISSN :
- 03636143 and 15221563
- Volume :
- 269
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- American Journal of Physiology - Cell Physiology
- Publication Type :
- Periodical
- Accession number :
- ejs66653283
- Full Text :
- https://doi.org/10.1152/ajpcell.1995.269.5.C1133