LOCATION OF THE LSP-1GENES IN DROSOPHILA SPECIES BY IN SITUHYBRIDIZATION

The locations of the larval serum protein one (LSP-1) α, β and γ genes were determined in Drosophila melanogasterand in 14 other species of Drosophila by in situhybridization to polytene chromosomes. The LSP-1α gene mapped to bands 11B on the X chromosome, the LSP-1β gene mapped to bands 21D-E on chromosome 2L, and the LSP-1γ gene mapped to band 61A in all the melanogaster subgroup species. In eight other species, both the LSP-1α and β genes mapped to one site on Muller's element E which corresponds to chromosome 3Rof D. melanogaster. No hybridization of LSP-1γ was detected in these eight species. Restriction enzyme digestion and analysis of genomic DNA by filter transfer hybridization confirmed the presence of LSP-1α-like and β-like genes in seven of these species. These results are discussed with respect to conservation of the chromosomal elements in the genus Drosophila.