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Pore-Forming Toxin-Driven Recovery of Peroxidase-Mimicking Activity in Biomass Channels for Label-Free Electrochemical Bacteria Sensing
- Source :
- Analytical Chemistry; May 2024, Vol. 96 Issue: 19 p7661-7668, 8p
- Publication Year :
- 2024
-
Abstract
- The development of sensitive, selective, and rapid methods to detect bacteria in complex media is essential to ensuring human health. Virulence factors, particularly pore-forming toxins (PFTs) secreted by pathogenic bacteria, play a crucial role in bacterial diseases and serve as indicators of disease severity. In this study, a nanochannel-based label-free electrochemical sensing platform was developed for the detection of specific pathogenic bacteria based on their secreted PFTs. In this design, wood substrate channels were functionalized with a Fe-based metal–organic framework (FeMOF) and then protected with a layer of phosphatidylcholine (PC)-based phospholipid membrane (PM) that serves as a peroxidase mimetic and a channel gatekeeper, respectively. Using Staphylococcus aureus(S. aureus) as the model bacteria, the PC-specific PFTs secreted by S. aureusperforate the PM layer. Now exposed to the FeMOF, uncharged 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) molecules in the electrolyte undergo oxidation to cationic products (ABTS•+). The measured transmembrane ionic current indicates the presence of S. aureusand methicillin-resistant S. aureus(MRSA) with a low detection limit of 3 cfu mL–1. Besides excellent specificity, this sensing approach exhibits satisfactory performance for the detection of target bacteria in the complex media of food.
Details
- Language :
- English
- ISSN :
- 00032700 and 15206882
- Volume :
- 96
- Issue :
- 19
- Database :
- Supplemental Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Periodical
- Accession number :
- ejs66387429
- Full Text :
- https://doi.org/10.1021/acs.analchem.4c00589