Back to Search
Start Over
Top-down mass spectrometry of native proteoforms and their complexes: a community study
- Source :
- Nature Methods; 20240101, Issue: Preprints p1-9, 9p
- Publication Year :
- 2024
-
Abstract
- The combination of native electrospray ionization with top-down fragmentation in mass spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and cofactors. Although this approach is powerful, both native MS and top-down MS are not yet well standardized, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics initiated a study to develop and test protocols for native MS combined with top-down fragmentation of proteins and protein complexes across 11 instruments in nine laboratories. Here we report the summary of the outcomes to provide robust benchmarks and a valuable entry point for the scientific community.
Details
- Language :
- English
- ISSN :
- 15487091 and 15487105
- Issue :
- Preprints
- Database :
- Supplemental Index
- Journal :
- Nature Methods
- Publication Type :
- Periodical
- Accession number :
- ejs66377168
- Full Text :
- https://doi.org/10.1038/s41592-024-02279-6