Back to Search
Start Over
Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose
- Source :
- FEBS Letters; January 1989, Vol. 244 Issue: 1 p127-131, 5p
- Publication Year :
- 1989
-
Abstract
- Endoglucanase CenA of Cellulomonas fimicomprises an N-terminal cellulose-binding domain and a C-terminal catalytic domain joined together by a sequence of 23 proline and threonine residues (the Pro-Thr box). The domains function independently when separated by proteolysis. Tn phoA has been used to generate cenA′-′ phoA fusions. CenA′-′PhoA fusion polypeptides which contain the entire cellulose-binding domain of CenA bind to cellulose, allowing their purification from periplasmic extracts in a single, facile step. This result has implications for purification or immobilisation of chimeric proteins on a cheap cellulose matrix.
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 244
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- FEBS Letters
- Publication Type :
- Periodical
- Accession number :
- ejs66348271
- Full Text :
- https://doi.org/10.1016/0014-5793(89)81177-9