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Expression of the Drosophila melanogastermetallothionein genes in yeast

Authors :
Silar, Philippe
Wegnez, Maurice
Source :
FEBS Letters; January 1990, Vol. 269 Issue: 1 p273-276, 4p
Publication Year :
1990

Abstract

The metallothionein system in Drosophilamelanogasteris composed of two genes, Mtoand Mn, that code for distinctly different proteins. In order to compare the properties of Mtoand Mtn, we transformed yeast with several fusion plasmids. The Mtoand MtncDNAs, when placed under the control of CUP1or PGKpromoters, can confer a copper-resistance phenotype to copper-hypersensitive cells. Both Mtoand Mtnproteins can be characterized in extracts from transformed yeast cells.

Details

Language :
English
ISSN :
00145793
Volume :
269
Issue :
1
Database :
Supplemental Index
Journal :
FEBS Letters
Publication Type :
Periodical
Accession number :
ejs66308706
Full Text :
https://doi.org/10.1016/0014-5793(90)81172-K