Precursor of the myo-inositol-binding protein of a Pseudomonasspecies

The biosynthesis of the periplasmic binding protein of the myo-inositol high affinity transport system in a Pseudomonasstrain involves the synthesis of a precursor of 2 kDa heavier than the mature form (30 kDa). The identification of the precursor is based on the comparison of the fingerprints of the two proteins and on kinetics in pulse-chase experiments.