Expression of the human apolipoprotein AI gene fused to the E. coligene for β-galactosidase

The human apoAI gene was expressed in E. coliby in-frame fusion to a modified β-galactosidase gene present in plasmid pUR291. The fused β-galactosidase-apoAI gene product was expressed at a high level and was recognized by an anti-human apoAI antiserum. Besides the fused protein, at least one degradation product having an Mrsimilar to that of β-galactosidase was present in high amounts in bacterial extracts. These results and those of a pulse-chase experiment indicate that degradation took place only in the apoAI moiety of the chimeric protein.