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The site of action of the A-chain of mistletoe lectin I on eukaryotic ribosomes The RNA N-glycosidase activity of the protein
- Source :
- FEBS Letters; January 1988, Vol. 231 Issue: 2 p378-380, 3p
- Publication Year :
- 1988
-
Abstract
- The site of action of the A-chain of mistletoe lectin (ML-A) from Viscum albumon eukaryotic ribosomes was studied. Treatment of rat liver ribosomes with ML-A, followed by treatment of the isolated rRNA with aniline, caused the release of a fragment with about 450 nucleotides from 28 S rRNA. Further analysis of nucleotide sequences of this fragment revealed that the aniline-sensitive site of phosphodiester bond was between positions A-4324 and G-4325 in 28 S rRNA. These results indicate that ML-A inactivates the ribosomes by cleaving a N-glycosidic bond at A-4324 of 28 S rRNA in the ribosomes as ricin A-chain does.
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 231
- Issue :
- 2
- Database :
- Supplemental Index
- Journal :
- FEBS Letters
- Publication Type :
- Periodical
- Accession number :
- ejs66299170
- Full Text :
- https://doi.org/10.1016/0014-5793(88)80853-6