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Development of Cell Culture Platforms for Study of Trabecular Meshwork Cells and Glaucoma Development

Authors :
Youn, Kook In
Lee, Ji Woo
Song, Youngjun
Lee, Sang Yeop
Song, Kwang Hoon
Source :
Tissue Engineering and Regenerative Medicine; 20240101, Issue: Preprints p1-16, 16p
Publication Year :
2024

Abstract

Background:: Various cell culture platforms that could display native environmental cue-mimicking stimuli were developed, and effects of environmental cues on cell behaviors were studied with the cell culture platforms. Likewise, various cell culture platforms mimicking native trabecular meshwork (TM) composed of juxtacanalicular, corneoscleral and uveal meshwork located in internal scleral sulcus were used to study effects of environmental cues and/or drug treatments on TM cells and glaucoma development. Glaucoma is a disease that could cause blindness, and cause of glaucoma is not clearly identified yet. It appears that aqueous humor (AH) outflow resistance increased by damages on pathway of AH outflow can elevate intraocular pressure (IOP). These overall possibly contribute to development of glaucoma. Methods:: For the study of glaucoma, static and dynamic cell culture platforms were developed. Particularly, the dynamic platforms exploiting AH outflow-mimicking perfusion or increased IOP-mimicking increased pressure were used to study how perfusion or increased pressure could affect TM cells. Overall, potential mechanisms of glaucoma development, TM structures and compositions, TM cell culture platform types and researches on TM cells and glaucoma development with the platforms were described in this review. Results and conclusion:: This will be useful to improve researches on TM cells and develop enhanced therapies targeting glaucoma.

Details

Language :
English
ISSN :
17382696 and 22125469
Issue :
Preprints
Database :
Supplemental Index
Journal :
Tissue Engineering and Regenerative Medicine
Publication Type :
Periodical
Accession number :
ejs66133396
Full Text :
https://doi.org/10.1007/s13770-024-00640-6