An 18-day, 3 °C cold treatment effectively kills Ceratitis capitata(Diptera: Tephritidae) in kiwifruit (Actinidiaspp.)

A series of experiments were carried out to develop a phytosanitary disinfestation protocol to kill Ceratitis capitata(Weidemann) (Mediterranean fruit fly, Diptera: Tephritidae) in ‘Hayward’ kiwifruit (Actinidia deliciosa(A. Chev.) C.F. Liang and A.R. Ferguson) and ‘Zesy002’ kiwifruit (Actinidia chinensisPlanch.). Experiments on 4 immature life stages (eggs and 3 larval instars) with treatment durations of between 5 and 18 days showed that third instars were the most tolerant to temperatures around 3 °C, with the lethal time to 99.9968% (probit 9) mortality (LT99.9968) estimated to be 17.3 days (95% confidence interval (CI) 16.4–18.2). Larvae reared and treated in ‘Zesy002’ were significantly more susceptible to cold treatment than those reared in ‘Hayward’. A large-scale trial testing a disinfestation protocol of 3 ± 0.5 °C for 18 days treated over 500,000 third-instar C. capitatawith no survivors. These results demonstrate that a cold treatment of 3.5 °C or below for 18 days induces C. capitatamortality in kiwifruit at a rate that exceeds 99.9968% with a degree of confidence greater than 99%.