Engineering of ketocarotenoid biosynthetic pathway in Chlamydomonas reinhardtiithrough exogenous gene expression

Graphical abstract: <fig id="Figa" position="anchor"> <graphic position="anchor" specific-use="HTML" mime-subtype="PNG" href="MediaObjects/43393_2024_240_Figa_HTML.png" id="MO1"></graphic> </fig>Graphical abstract for the study carried out which represents the in silicoplasmid vector designing, algae transformation by electroporation, selection on antibiotic plates, PCR amplification for GM confirmation, Southern hybridization to confirm gene integration, Western blotting to check protein expression, pigment quantification, and algae growth determination.