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Using MitER for 3D analysis of mitochondrial morphology and ER contacts
- Source :
- Cell Reports Methods; January 2024, Vol. 4 Issue: 1
- Publication Year :
- 2024
-
Abstract
- We have developed an open-source workflow that allows for quantitative single-cell analysis of organelle morphology, distribution, and inter-organelle contacts with an emphasis on the analysis of mitochondria and mitochondria-endoplasmic reticulum (mito-ER) contact sites. As the importance of inter-organelle contacts becomes more widely recognized, there is a concomitant increase in demand for tools to analyze subcellular architecture. Here, we describe a workflow we call MitER (pronounced “mightier”), which allows for automated calculation of organelle morphology, distribution, and inter-organelle contacts from 3D renderings by employing the animation software Blender. We then use MitER to quantify the variations in the mito-ER networks of Saccharomyces cerevisiae, revealing significantly more mito-ER contacts within respiring cells compared to fermenting cells. We then demonstrate how this workflow can be applied to mammalian systems and used to monitor mitochondrial dynamics and inter-organelle contact in time-lapse studies.
Details
- Language :
- English
- ISSN :
- 26672375
- Volume :
- 4
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Cell Reports Methods
- Publication Type :
- Periodical
- Accession number :
- ejs65239831
- Full Text :
- https://doi.org/10.1016/j.crmeth.2023.100692