Synergistic antibacterial effect against S. Aureusin combination with amoxicillin using fluconazole nanoparticles studied under atomic force microscopy

COVID-19 patients are increasing day by day. The risk of bacteria-virus co-infection also escalates. Antibiotic resistance caused by pathogenic bacteria has become a major health challenge these days. The key objective was to examine the antibacterial effects of Fluconazole coated silver nanoparticles (Ful-AgNPs) in combination with amoxicillin against bacteria that show resistance to amoxicillin. Here we report quick, and feasible synthetic procedure for the preparation of Ful-AgNPs. Characterisation of nanoparticles was performed by employing Uv, FTIR, and AFM techniques. The size of Ful-AgNPs found in the range from 9-18 nm. Fractional inhibitory concentration index showed synergistic interaction of Ful-AgNPs with amoxicillin with effective inhibitory values found to be 25-50 μg while MIC for amoxicillin alone was found to be 75 μg. Bioassay conducted via AFM along with classical bioassay that supports the synergistic effect of Ful-AgNPs on the antibacterial activity of amoxicillin. Synthesised nanoparticles have found nontoxic against human mononuclear macrophages.